Identification and Functional Characterization of the RcFAH12 Promoter from Castor Bean in Arabidopsis thaliana

Castor (Ricinus communis L.) seed oil is the commercial source of ricinoleate, a valuable raw material used in many industries. Oleoyl-12-hydroxylase (RcFAH12) key enzyme biosynthesis accumulating nearly 90% triacylglycerol castor seeds. Little known about transcriptional regulation RcFAH12. We rapid amplification cDNA 5′ ends (5′RACE) to locate transcription start site (TSS) RcFAH12, and sequence 2605 bp region, −2506~+99, surrounding TSS was cloned. then investigated these regions promote β-glucuronidase (GUS) expression transgenic Arabidopsis by progressive 3′ deletions strategies. The GUS staining showed that accumulation varied tissues under control different deleted fragments In addition, driven RcFAH12 promoter markedly accumulated seeds, which indicated might play an important role ricinoleic acid. This study will lay potential foundation for developing tissue-specific oil-seed crops.